random mutagenesis meaning in English
无规突变诱变
Examples
- The mutant pel - d92l was expressed in pichia pastoris gs115 , sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs , and its " yield decreased dramatically , the themostability of pel - d92l - gs is also different from the pel - gs , but their optimum temperatures are same . 3 . directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel , using the oligos " beginning " and " end " , homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的随机诱变用易错pcr方法对pel基因进行随机诱变， pcr产物与ppic3 . 5k连接，转化大肠杆菌，获得的混合质粒电转化毕赤酵母gs115 ， omm平板筛选适于低温或对热稳定的重组子，筛选获得一株最活反应温度、热稳定性、发酵酶活均有提高的突变体pel - ep5 - gs ，其最活反应温度为45 ，比野生型高出5 ； 40处理30min残留活性为56 ，大大高于野生型的6 ；初始ph7 . 3528条件下培养72h ，发酵上清酶活为325u / ml 。